Molecular Characterization of Cissus Quadrangularis Linn Using Pseudomonas Aeruginosa
The present paper deals with the molecular assay of Cissus quadrangularis Linn. which is commonly known as a "bone setter". The plant was inoculated with pathogen Pseudomonas aeruginosa at different concentration of inoculam (0.5 ml, 1 ml and 1.5ml ) and at different time interval (24, 48 and 72 hours) .The plant parts were cut, dried and powdered. The powdered samples were taken for molecular assay. The molecular characterization of DNA showed that the number of bands increases with time in each of the species and are found to be more when compared to control readings. The most prominent effect in increase of the characterization was seen with Pseudomonas species. Comparative study of the molecular assay of DNA profile of the control and the treated one was done to locate the specific site of DNA that could be targeted for Drug designing in future.